Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 9;9:11571. doi: 10.1038/s41598-019-47964-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(a) Schematic representation of the Tyr locus. The target sequence of Tyr exon 2 recognized by gRNA is overlined and the protospacer adjacent motif (PAM) sequence is marked in red. The target nucleotide ‘G’ marked in green is a key nucleotide for tyrosinase activity, and nucleotide replacement at this position often leads to an albino phenotype. PCR primers used to evaluate the indel mutations around the target site are indicated as red arrows. (b) BN offspring obtained after electroporation of CRISPR/Cas9 complex in IVF embryos. By contrast with the normal (dark brown) BN rats (left panel), all of the offspring obtained had the albino phenotype (right panel). (c) PCR products amplified from genomic DNA of offspring with the albino phenotype represented in (b) showed a wide variety of band patterns. (d) Representative Sanger sequencing at the gRNA target site of male-4 (♂ 4) and female-7 (♀ 7). (e) Sequence analysis of PCR products amplified from the genomic DNA of offspring with the albino phenotype represented in (b). All of the sequences examined contained a wide variety of biallelic indel mutations (green). Insertions are underlined. n.a.: not amplified due to problems with PCR.