Table 5.
Knock-in efficiency of rat offspring after zygote genome editing using electroporation followed by AAV transduction.
| Target gene |
No. of embryos electroporated |
No. of embryos cultured with AAV |
No. of embryos survived (%) |
No. of embryos transferred | No. of offspring (%) |
No. of KO offspring without GFP signal (%) |
No. of offspring with GFP signal (%) |
No. of offspring with partial insertion (%) |
No. of offspring with random integration (%) |
|---|---|---|---|---|---|---|---|---|---|
| Rosa 26 | 376 | 366 | 349 (95.4) | 180 | 19 (10.6) | 7 (36.8) | 12 (63.2) | 9 (47.4) | 2 (10.5) |