Fig. 1.

TAZ regulates SOX2 expression in HNSCC cells. a Reduced SOX2 expression upon shRNA-mediated TAZ knockdown was measured in both Cal27 and Fadu cells by western bot (left panel) and qRT-PCR (right panel) assays. b Enforced TAZ overexpression resulted in SOX2 upregulation in HN6 and Fadu cells. c Reduced SOX2 expression upon siRNA-mediated TEAD4 knockdown was measured in both Cal27 and Fadu by western bot (left panel) and qRT-PCR (right panel) assays. d Bioinformatics prediction of 8 potential binding sites of TEAD4 in human SOX2 promoter region via online JASPAR database (http://jaspar.genereg.net/). Data were presented as Mean ± SD from three independent experiments, *P < 0.05, **P < 0.01