Fig. 1. Structure of optineurin.

Graphic illustration of optineurin at genomic (a), mRNA (b), and protein levels (c, e). a The OPTN structure at genomic level was generated using the NCBI sequence (reference NG_012876.1 (https://www.ncbi.nlm.nih.gov/nuccore/257743476)), and the scaling factors used were 1:400 bp (for exons) and 1:4000 bp (for introns). Together, 16 exons form the OPTN gene. b The mRNA was generated from the same source and consists of 1734 bp. c The protein contains various structural domains, and the localization of these domains is indicated relative to the amino acid sequence as well as to some interacting proteins (d). LZ Leucine-zipper domain, LIR LC3-interacting region, CC coiled coil domain, UBD ubiquitin-binding domain, ZF zinc finger domain, TBK tank binding kinase, Rab8 RAB8A, Member RAS Oncogene Family, LC3/GABARAP light chain 3/beta, GABA type A receptor associated protein. CYLD CYLD Lysine 63 Deubiquitinase, HTT huntingtin. e Crystal structure of different OPTN domains as determined by X-ray analysis. These images were made with VMD, which is owned by the theoretical and computational biophysics group, NIH Center for Macromolecular Modeling and Bioinformatics, at the Beckman Institute, University of Illinois at Urbana-Champaign. E.1¹⁴ represents the structure of the amino acids 26–103 (HLAHP NLDTFTPEEL LQQMKELLTE NHQLKEAMKL NNQAMKGRFE ELSAWTEKQK EERQFFEIQS KEAKERLMAL SHE). E.2¹⁵ represents OPTN amino acids from 170 to 181 or LC3B interacting region (the sequence consists of SGSSEDSFVE I). E.3¹⁶ shows the crystal structure of UBD spanning the amino acids 416–510 (EKVDR AVLKELSEKL ELAEKALASK QLQMDEMKQ TIAKQEEDLE TMTILRAQME VYCSDFHAER AAREKIHEEK EQLALQL AVL LKENDAFEDG)