Fig. 6.

ABI5 expression is activated in the presence of ABF3 and 14-3-3 as measured by the transactivation assay. Schematic diagrams of the reporter, effector, and internal control plasmids of the mesophyll protoplasts used in transient transactivation assays. a The reporter plasmid contained the ABI5 promoter (2055 bp). The effector plasmids contained the ABF3 and 14-3-3ω gene, which were driven by the CaMV35S promoter. The PRL vector contained a CaMV35S promoter driving the Renilla luciferase gene, Luc, as an internal control. b The results showed that ABI5 is regulated by ABF3 and 14-3-3ω. Data are presented as the mean ± standard deviation (SD). N = 3, three biological repeats with five technical replicates for each biological repeat. Relative activity is calculated as the LUC firefly/LUC Renilla but is normalized to the reporter only. The fold change of the relative activity significantly differed from the reporter only as indicated by *P < 0.05 using the Student’s T-test