Fig. 7.

The ABI5 promoter deletion assay. Schematic diagrams of the reporter, effector, and internal control plasmids used in the transient transactivation assay in Arabidopsis leaf protoplasts. a The reporter plasmid contained the ABI5 promoter. For the effector plasmid, the ABF3 gene was driven by the CaMV35S promoter. The PRL vector contained a CaMV35S promoter that drove the Renilla luciferase gene, Luc, as an internal control. b A schematic diagram of constructs of the ABI5 promoter with 5′ end deletions fused to the Luc reporter gene. D1 contains an ABRE cis-element (BOX 1 to BOX 5). D2 contains an ABRE cis-element (BOX 1 to BOX 4). D3 contains an ABRE cis-element (BOX 1 to BOX 3). D4 contains an ABRE cis-element (BOX 1). c The results indicated that through the deletion of the ABI5 promoter, transcriptional activity was decreased. ABF3 bound to BOX4 and BOX5 of the ABRE cis-element to regulate ABI5 transcription. Data are presented as the mean ± standard error (SE). N = 3, three biological repeats with three technical replicates for each biological repeat. The relative activity is calculated as the LUC firefly/LUC Renilla but is normalized to the minus ABF effector. The relative activity fold-change significantly differed from D1 is indicated by *P < 0.05 using the Student’s T-test