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. 2019 Aug 9;9:11584. doi: 10.1038/s41598-019-48095-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometric analysis of tetraspanin CD81, CD63 and CD9 expression in extracellular vesicles (EVs) subtypes isolated from pig seminal plasma. (a) Histogram representative of CD81/CD63/CD9 expression in EV subtypes (exosomes and microvesicles). Fluorescence (CD81-APC-A, CD63-KO525-A and CD9-PE, x-axis), vs number of events (Count, y-axis). (b) Box-whisker plot showing variation in CD81/CD63/CD9 expression between EV subtypes of 12 ejaculates (one per boar). Boxes enclose the 25^th and 75^th percentiles; the line is the median; and the whiskers extend to the 5^th and 95^th percentiles. (a,b) and (x–y) indicate significant differences (P < 0.001 and P < 0.05, respectively) among the EV subtypes.