Fig. 5.

Conformational changes upon E123IR removal from Atg3. a Crystal structure of Atg3^∆NFR (PDB 6OJJ, from this study). b Superposition of Atg3^∆NFR (PDB 6OJJ, light blue with catalytic Cys shown in green) with prior structure of Atg3^FL (cyan, PDB 2DYT) with E123IR and catalytic Cys shown in red, and differences highlighted in cartoons. c Fo–Fc map shown at 3σ after omitting the catalytic cysteine region (residues 231–237) of Atg3^∆NFR and performing simulated annealing. d Close-ups of catalytic elements from Atg3^∆NFR and Atg3^FL. e Close-up superposition of Atg3^∆NFR (light blue) and Atg3^FL (cyan) structures, showing interactions between Atg3′s catalytic domain and E123IR, and conformational rearrangements upon E123IR dislocation