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. 2019 Aug 9;10:3611. doi: 10.1038/s41467-019-11538-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The methyl group carbon C25 arises from C2 of acetate. Comparison of ¹³C NMR spectra for 2 isolated after growth in the presence of [1,2-¹³C₂] sodium acetate. Integration of the ¹³C NMR spectra confirmed weak enrichment of the ¹³C isotope and the absence of coupling to any adjacent carbon atom. In contrast, the methyl group atom C24 shows enrichment and coupling to C1. The C2 atom of [1,2-¹³C₂] sodium acetate is highlighted as a blue circle, the C1 atom as a black circle, and the coupled unit by a bold line