Table 3.
Production of PDO from (D/L)-DHB by E. coli MG1655 wild-type (wt) and derived host strains harboring PDO downstream pathway.
| Strain | Genes expressed | (D/L)-DHB consumed [mM] | (L)-DHB consumed [mM] | PDO produced [mM] | PDO conversion from L-DHB [%] |
|---|---|---|---|---|---|
| Pen946 | pACT3 | 9.9 (±2.8) | 9.4 (±2.9) | 0.0 (±0.0) | 0 |
| Pen911 | pACT3-llDdV108C-kdcA-yqhD | 11.9 (±1.2) | 11.9 (±1.2) | 2.0 (±0.4) | 17 |
| Pen913 | pACT3-llDdV108C-kdcAV461I-yqhD | 11.9 (±1.9) | 9.9 (±1.0) | 5.5 (±0.5) | 55 |
| Pen965 | pACT3-llDdV108C-pdc-yqhD | 11.6 (±4.2) | 10.8 (±4.4) | 1.1 (±0.5) | 11 |
| Pen966 | pACT3-llDdV108C-pdcW392Q-yqhD | 11.1 (±0.04) | 6.4 (±0.7) | 7.1 (±0.3) | 110 |
Cells were cultivated in 250 mL non-baffled shake flasks on mineral medium containing 20 g L−1 glucose. At OD600 ~0.6, IPTG (1 mM) and a racemic mixture of (D/L)-DHB (50 mM) were added to the medium. After 24 h of cell cultivation, PDO and (D/L)-DHB titers were measured. Reported data are the mean (±SD) of three independent replicates.