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. 2019 Aug 9;9:11579. doi: 10.1038/s41598-019-47831-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MMP12 inhibits expression of CCL2 and CCR2 following chemical corneal injury. (A–C) Relative expression levels of CCL2 mRNA in unwounded corneas and chemically wounded corneas of WT and Mmp12^−/− mice, as determined by qRT-PCR. Expression levels are relative to uninjured WT corneas. Results are relative expression levels (means ± s.e.m.) at time points unwounded (Un; WT N = 12; KO N = 10), 1 day (1D; WT N = 12, KO N = 12), 4 days (4D; WT N = 4; KO N = 4), and 6 days (6D; WT N = 12; KO N = 12) after injury. (D–F) Relative expression levels of CCR2 mRNA in unwounded corneas and chemically wounded corneas of WT and Mmp12^−/− mice, as determined by qRT-PCR. Expression levels are relative to uninjured WT corneas. Results are relative expression levels (means ± s.e.m.) at time points unwounded (Un; WT N = 6; KO N = 6), 1 day (1D; WT N = 6, KO N = 6), 4 days (4D; WT N = 4; KO N = 4), and 6 days (6D; WT N = 6; KO N = 6) after injury. ****P < 0.0001, **P < 0.05 and *P < 0.05.