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. 2019 Aug 9;9:11579. doi: 10.1038/s41598-019-47831-z

Figure 3.

Figure 3

Temporal expression of CCL2 and CCR2 in epithelial injured corneas of WT and MMP12 KO mice. (A–C) Relative expression levels of CCL2 mRNA in unwounded corneas and epithelial wounded corneas of WT and Mmp12−/− mice, as determined by qRT-PCR. Expression levels are relative to uninjured WT corneas. Results are relative expression levels (means ± s.e.m.) at time points unwounded (Un; WT N = 12; KO N = 10), 1 hour (1 H, WT N = 15; KO N = 3), 2 hours (2 H, WT N = 15; KO N = 4), 16 hours (16 H, WT N = 3; KO N = 3), 1 day (1D; WT N = 3, KO N = 3), 2 days (2D; WT N = 14; KO N = 3), 4 days (4D; WT N = 3; KO N = 5), and 6 days (6D; WT N = 5; KO N = 7) after injury. (D–F) Relative expression levels of CCR2 mRNA in unwounded corneas and epithelial wounded corneas of WT and Mmp12−/− mice, as determined by qRT-PCR. Expression levels are relative to uninjured WT corneas. Results are relative expression levels (means ± s.e.m.) at time points unwounded (Un; WT N = 6; KO N = 10), 1 hour (1 H, WT N = 9; KO N = 3), 2 hours (2 H, WT N = 9; KO N = 4), 16 hours (16 H, WT N = 3; KO N = 3), 1 day (1D; WT N = 3, KO N = 3), 2 days (2D; WT N = 8; KO N = 3), 4 days (4D; WT N = 3; KO N = 5), and 6 days (6D; WT N = 5; KO N = 7) after injury. ****P < 0.0001, ***P < 0.005, **P < 0.05 and *P < 0.05.