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. 2019 Aug 9;10:3597. doi: 10.1038/s41467-019-11416-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Expression of S1+55 variants and vvl1+2 in wild type and mutant backgrounds. Expression of S1+55 class 2 reporter (a, left panel) compared to that of vvl1+2 (a, right panel) in wild type, Dfd Scr (b) or hth^P2 (c) mutant embryos. Asterisks mark the segments where S1+55 expression is lost. Expression of S1+55cl3 class 3 containing reporter (d, left panel) compared to that of vvl1+2 in wild type (d, right panel), and its expression in Scr Antp Ubx (e) or hth^P2 (f) mutant embryos. Yellow arrows mark the segments where S1+55cl3 expression is lost. Note the maxillary expression in e is in the Dfd domain, which should not be affected in this genotype. Expression of S1+55cl1 class 1 reporter (g, left panel) compared to that of vvl1+2 in wild type (g, right panel). Blue arrow marks the additional intercalary (ic) segment patch where vvl1+2 is not expressed. h Close-up of S1+55cl1 expression (green) in the intercalary segment stained with anti-Lab antibody (purple) compared to vvl1+2 (red) expression. Intercalary S1+55cl1 expression is lost in lab mutant (i) or hth^P2 (j) mutant embryos. k Expression of S1+55cl2OF class 2 optimal flanks reporter compared to that of vvl1+2 in wild type (k, right panel); Dfd Scr (l) or hth^P2 (m) mutant embryos. Arrowhead in (k, right panel) points to expression outside the normal vvl1+2 domain. Asterisks in l–m mark the segments where S1+55cl2OF expression is lost. n Expression of S1+55cl3OF reporter compared to that of vvl1+2 in wild type (n, right panel); Scr Antp Ubx (o) or hth^P2 (p) mutant embryos. Yellow arrows mark the segments where S1+55cl3OF expression is lost. q Expression of S1+55cl1OF reporter compared to that of vvl1+2 in wild type (q, right panel). Blue arrow marks the intercalary segment not expressing vvl1+2 and exhibiting extended Lab expression. Intercalary S1+55cl1OF expression is lost in lab (r) or hth^P2 (s) mutant embryos. Expression of S1+55mut (t), a Hox-cofactor mutant site reporter, compared to that of vvl1+2 in wild type embryos (t, right panel). White scale bar 50 μm. Blue scale bar 25 μm. All embryos shown in these and following figures are at stage 11 (st11)