Table 2.
Primers and probes used in this study
| Type | Name | Sequence (5′–3′)a | Tm (°C)b | Reference |
|---|---|---|---|---|
| F | HAV68 | TCACCGCCGTTTGCCTAG | 59.1 | Costafreda et al. (2006) |
| P | HAV150 | [FAM]-CCTGAACCTGCAGGAATTAA-[MGB][EQ] | Not assessed | Costafreda et al. (2006) |
| R | HAV240 | GGAGAGCCCTGGAAGAAAG | 57.0 | Costafreda et al. (2006) |
| R | PM A | GGAGAGCCCTGGAAGAAAA | 56.4 | This study |
| R | PM B | GGAGAGCCCTGGAAAAGAG | 57.0 | This study |
| R | PM C | GGAGAGCCCTGGAAGAAGA | 58.1 | This study |
| R | PM D | GGAGAGCCCTGGAAAAATA | 53.7 | This study |
FAM 6-carboxyfluorescein, MGB Minor Groove Binder, EQ eclipse quencher
aDifferences from the original primers are displayed in italics
bCalculated by OligoAnalyzer 3.1 (Integrated DNA Technologies)