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Estimated effects of reverse primer mismatches (obtained from Model 1)
| RNA | DNA | |||||
|---|---|---|---|---|---|---|
| RT-ddPCR | RT-qPCR | RT-qPCR, dd-kit | ddPCR | qPCR | qPCR, dd-kit | |
| Degree of underestimation [log10 difference from PM (times underestimation on the linear scale)] | − 0.54 (3.5) | − 1.10 (12.6) | − 0.54 (3.5) | − 1.5 (29) | − 2.5 (295) | − 2.4 (229) |
The value in each column describes the expected difference in concentration when using the MM under the setting given by the column headers, across the four strains tested
