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. 2019 Aug 5;11:7369–7376. doi: 10.2147/CMAR.S211020

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© 2019 Li et al.

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The expression of hsa_circ_0085616 in the Hep3B cell line was upregulated by plasmid. (A) Hep3B cells transfected with plasmid had significantly increased expression of hsa_circ_0085616 compared with the negative control. (B) The proliferation of Hep3B cells increased when cells showed overexpression of hsa_circ_0085616. (C) The transwell assay showed that the migration and invasion of transfected Hep3B cells were promoted when compared to the negative control. (D) Flow cytometry results showed that more Hep3B-transfected cells stayed at S and G2/M stage than G1/G0 stage. (E) Flow cytometry results showed that Hep3B cell apoptosis decreased after transfection with plasmid. **p<0.01 and ****p<0.0001 compared to controls.

Abbreviation: NC, Negative control.