Table 1.
Primer sets for RT-PCR analysis assay
| Genes | Forward | Reverse |
|---|---|---|
| IL-6 (h) | 5ʹ-CCTGAACCTTCCAAAGATGGC-3’ | 5ʹ-TTCACCAGGCAAGTCTCCTCA-3’ |
| IL-8 (h) | 5ʹACTGAGAGTGATTGAGAGTGGAC-3’ | 5ʹAACCCTCTGCACCCAGTTTTC-3’ |
| IL-17A (h) | 5ʹ-TCCCACGAAATCCAGGATGCC-3’ | 5ʹ-CACAGTGGTCCTTCCAGGTT-3’ |
| MUC5AC (h) | 5ʹ-CTTACTCCACCCAAACCTGC-3’ | 5ʹ-CTGTCAACCCCTCTGACCAC-3’ |
| SIRT1 (h) | 5ʹ-GGAGGAGGCATTCGGAAAG-3’ | 5ʹ-TCGTTGGTGTCACACAGAT-3’ |
| GAPDH (h) | 5ʹ-TGTTGCCATCAATGACCCCTT-3’ | 5ʹ-CTCCACGACGTACTCAGCG-3’ |
| IL-6 (m) | 5ʹ-TCTATACCACTTCACAAGTCGGA-3’ | 5ʹ-GAATTGCCATTGCACAACTCTTT-3’ |
| IL-8 (m) | 5ʹ-AAGGCTGGTCCATGCTCT-3’ | 5ʹ-CACAGACATCGTAGCTCTTGAGTG-3’ |
| IL-17A (m) | 5ʹ-TCAGCGTGTCCAAACACTGAG-3’ | 5ʹ-CGCCAAGGGAGTTAAAGACTT-3’ |
| MUC5AC (m) | 5ʹ-CTGTGACATTATCCCATAAGCCC-3’ | 5ʹ-AAGGGGTATAGCTGGCCTGA-3’ |
| SIRT1 (m) | 5ʹ-CACTGTAACTGGGGGCAACT-3’ | 5ʹ-CACTTCTTGTCAGCGTCGAA-3’ |
Abbreviations: h, Human; m, Mouse.