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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Thromb Haemost. 2019 Jun 20;17(8):1384–1396. doi: 10.1111/jth.14521

Figure 2. SCARA5 expressing cells bind and internalize VWF-FVIII.

Figure 2

HEK 293T cells were transiently transfected with the full-length human SCARA5 cDNA. 24 hours post transfection cells were exposed to human pdVWF for 15–60 minutes, washed, and processed for immunocytochemistry. (A) Human pdVWF bound to and internalized by HEK 293T cells transiently expressing human SCARA5. (B) Human pdVWF internalized by HEK 293T cells transiently expressing human SCARA5 partially co-localized with EEA1 after 15 minutes. (C) Human pdVWF internalized by HEK 293T cells transiently expressing human SCARA5 partially co-localized with human FVIII. For merged images an orthogonal view is inset. For all images, DAPI = blue.