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. 2019 Aug 2;25:5747–5756. doi: 10.12659/MSM.914555

Figure 3.

Figure 3

Prediction and validation of Let-7g-5p potential target genes and the effects on SUNE1 cells. (A) Searching for the target gene of Let-7g-5p in the database TargetScan, miRDB, TargetMiner. (B) Dual-luciferase reporter assay was used to verify that Let-7g-5p directly regulated IGF-1R expression by 3′UTR. (C–E) RT-qPCR and western blot were used to detect the expression levels of IGF-1R mRNA and protein in the 5 groups. (F) CCK-8 assay was used to detect the cell viability in the 5 groups. * P<0.05, ** P<0.01, versus control group; # P<0.05, ## P<0.01, versus Let-7g-5p mimics group; ^ P<0.05, ^^ P<0.01, versus IGF-1R group. RT-qPCR – real-time quantitative polymerase chain reaction; CCK-8 – cell counting kit-8.