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Overlaid MALDI-MS spectra of affibodies (black, WT; blue, N23BP; red, Z) after conjugation with maleimide-benzophenone. Cysteine-containing affibodies show near complete conjugation with one maleimide-benzophenone, and smaller peaks show a second conjugation, likely with a lysine residue. Labels indicate the expected mass of each affibody.
