FIG 2.

LED209 blocking of QseC during in vivo infection decreases the expression levels of qseC gene in O104:H4 Stx⁺ and Stx^– strains. (A and B) qRT-PCR analyses of qseC from feces collected from days 1 to 10 p.i. of both C227-11 and BA3826 strains. (C) qRT-PCR of qseC with RNA extracted from cultures of EHEC 86-42, C227-11 (Stx⁺), BA3826 (Stx^–), 17-2, and 042 strains grown in LB medium until reaching an OD₆₀₀ of 1.0. A Student t test was used to determine the statistical significance between treated and nontreated groups, or ANOVA was used for comparisons to EHEC 86-24. Error bars indicate the SD of the mean (**, P < 0.001; ***, P < 0.0001; ns, not significant).