TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| 86-24 | EHEC O157:H7 LEE and Stx2a (isolated from outbreak of HUS in the USA) | 95 |
| WT C227-11 | WT EAEC O104:H4 pAA AggR AAF/I Stx2a+ Pic SigA SepA (isolated from German outbreak in 2011) | 58 |
| C227-11::qseC | EAEC O104:H4 qseC mutant | This study |
| C227-11::qseC+ | EAEC O104:H4 qseC (pBAD33) complemented strain | This study |
| BA3826 | EAEC O104:H4 pAA, AggR, AAF/III, Pic, EAST-1 (isolated from a case-control study in Brazil) | 13 |
| 042 | EAEC O44:H18 pAA, AggR, AAF/II, Pet, Pic, EAST-1 (isolated from a case of diarrhea in Peru) | 96 |
| 17-2 | EAEC O3:H2 pAA, AggR, AAF/I, EAST-1 (isolated from a case of diarrhea in Chile) | 97 |
| DH5α | E. coli supE44 ΔlacU169(ϕ80lacZΔM15) hsdR17 | Stratagene |
| DH5α(λpir) | DH5α transduced with λpir; Nalr | 98 |
| S17-1(λpir) | Pro Res– mod+ RP4-2 Tc::UM-Km::Tn7 Strr | 99 |
| TOP10 | E. coli F– mcrAΔ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recA1 araD139Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| Plasmids | ||
| pJP5603 | 3.1-kb R6K-based suicide vector; Kmr | 92 |
| pBAD33 | Low-copy-number expression vector; Cmr | 100 |
a
LEE, locus of enterocyte effacement; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; Strr, streptomycin resistance; Nalr, nalidixic acid resistance. AAF/I, pilin subunit of aggregative adherence fimbriae I; SigA, Shigella IgA-like protease homologue; SepA, Shigella extracellular protein A; AAF/III, pilin subunit of aggregative adherence fimbriae III; EAST-1, EAEC heat-stable enterotoxin 1; AAF/II, pilin subunit of aggregative adherence fimbriae II; Pet, protease involved in intestinal colonization.