FIG 6.
Genetic complementation of yejM1163. The ΔtonB and ΔtonB yejM1163 mutant strains containing an empty vector plasmid (pBAD24) or a yejM clone (pBAD24-yejM) were grown on LBA plus ampicillin (A) or LBA plus ampicillin and ferric chloride (B). Growth was recorded after incubation at 37°C for 24 h. The bacterial strains used are as follows: 1a and b, RAM3144 [ΔtonB::scar(pBAD24)]; 2a and b, RAM3145 [ΔtonB::scar(pBAD24-yejM)]; 3a and b, RAM3146 [ΔtonB::scar yejM1163(pBAD24)]; and 4a and b, RAM3147 [(ΔtonB::scar yejM1163(pBAD24-yejM)].