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. 2019 Aug 10;18:131. doi: 10.1186/s12934-019-1182-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Overexpression of membrane proteins in E. coli C41(DE3) (a), C41(DE3)∆(ompF) (b) and C41(DE3)∆(ompF-acrAB) (c). Equal amounts (50 µg) of isolated membranes were loaded on 10% SDS-PAGE and stained with Coomassie Brilliant Blue. Target proteins are marked by red boxes. Independent expressions were performed three times with similar results