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. 2019 Aug 10;18:131. doi: 10.1186/s12934-019-1182-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Coomassie Brilliant Blue stained 10% SDS-PAGE of the purification of HlyB from E. coli C41(DE3)∆(ompF-acrAB) employing different detergent-combinations for solubilization (first detergent) and subsequent IMAC (second detergent). (1) Fos-choline 14/DDM, (2) DDM/DDM, (3) TX-100/DDM. Shown are the pooled eluate fractions of equal volume. Independent expressions and purifications were performed two times with similar results