Figure 10. Effect of synthesized calcium phosphate (apatite containing carbonic acid) on the adhesion/proliferation of MC3T3-E1 cells. (A) Phase contrast image. Shape of apatite containing carbonic acid (upper panel), and MC3T3-E1 cells cultured on fibronectin-coated plates (lower panel). Lower panels show the image of fibronectin-coated apatite. (B) SEM images (×1000) of HA-coated plate and fibronectin-coated plate (lower panel). (C) SEM images (×1000) of cells cultured on normal culture dish. (D) Proliferation of MC3T3-E1 cells was evaluated by the WST-1 assay. Values are mean±SE for 5 wells. The reproducibility was confirmed by one additional experiment. ***p<0.001 vs. the corresponding control group.