Figure 6. Proliferation of MC3T3-E1 cells on sintering Ti plate containing HA. (A) SEM image (×100, ×300) of cells on the Ti plate. (B) SEM images (×1000) show the surface shape of Ti plate containing HA (upper panels), and adhesive cells on the plate (lower panels). (C) The proliferation of MC3T3-E1 cells on plates was quantitatively evaluated by the WST-1 assay. White area in columns represents O2– production and black columns represent proliferation of cells. Values are mean±SE for 5 wells. The reproducibility was confirmed by one additional experiment. *p<0.05, **p<0.01, ***p<0.001 vs. the corresponding control group. (D) SEM image (x300, x1000) of cells on the fibronectin-coated Ti plate.