Figure 4. Effect of FN on stem cell-like phenotype and self-renewal transcription factors in DPCs. Cells were cultured with various concentrations of FN (10-100 μM) for 24 h. (a and b) After treatment, the levels of integrin β1, CD44, Nanog, Sox-2 were analyzed by western blot. β-actin was served as loading control. The immunoblot signals were quantified by densitometry. The data represent the means of three independent samples±SD. *p<0.05 versus non-treated control. (c and d) Expression of Nanog and Sox-2 was analyzed by immunofluorescence staining.
