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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Neurobiol Dis. 2019 Jun 15;130:104508. doi: 10.1016/j.nbd.2019.104508

Figure 1: Antagonizing miR-324-5p increases hippocampal A-type potassium currents in mice.

Figure 1:

(A) Timeline depicting ICV antagomir injection, shipping and patch clamp recording for potassium current measurement. (B) Representative traces showing A-type potassium current recorded from a CA1 pyramidal neuron from mice injected with either scrambled or miR-324-5p antagomir. (C) Summary plot showing that the peak IKA was significantly greater at the most depolarized test voltages in mice treated with the antagomir compared to scrambled (SCR: n=9 from 3 mice; a-324-5p: n=7 from 3 mice; 2-way RM ANOVA with Sidak’s multiple comparison post hoc testing, interaction antagomir X membrane potential: F(16,224)=3.194, p<0.0001; *p=0.004 at VM = 50, and *p=0.005 at VM = 60 mV). (D) The maximum IKA current density was significantly greater in mice treated with antagomir compared to scrambled (SCR: n=20 from 9 mice; a-324-5p: n=16 from 9 mice; unpaired two-tailed Mann-Whitney test, *p=0.036). (E) There was no difference in the voltage-dependence of activation of IKA between scrambled and miR-324-5p antagomir-injected mice (n=8 from 3 mice each). (F,G) There was no significant difference in either the slowly inactivating (F: SCR: n=8 from 9 mice; a-324-5p: n=7 from 9 mice; unpaired two-tailed t-test, t(13)=1.032, p=0.321) or sustained (G: SCR: n=20 from 9 mice; a-324-5p: n=15 from 9 mice; two-tailed Mann-Whitney test, p=0.382) potassium currents between neurons from miR-324-5p antagomir- and scrambled antagomir-injected mice. Error bars represent SEM. Additional analyses shown in Fig. S1.