Table 3.
Changes in PDK4 gene hypomethylation before and after TKA/UKA surgery and exercise therapy in elderly patients with end-stage knee osteoarthritis
| CpG island (position) (%) | Pre | Post | P-value |
|---|---|---|---|
| 185bp (upstream) | 83.67±2.23 | 78.83±1.26 | 0.028* |
| 188bp (downstream) | 18.55±1.05 | 15.94±0.95 | 0.046* |
| 205bp (downstream) | 26.80±0.34 | 24.22±0.74 | 0.027* |
| 228bp (downstream) | 9.23±0.28 | 8.07±0.42 | 0.028* |
| 261bp (downstream) | 4.88±0.51 | 4.07±0.66 | 0.046* |
| 317bp (downstream) | 8.21±0.61 | 7.67±0.24 | 0.046* |
| 366bp (downstream) | 6.68±0.66 | 4.29±0.72 | 0.028* |
| 420bp (downstream) | 70.74±2.03 | 65.84±1.70 | 0.028* |
| Promoter region | 2.19±3.52 | 0.31±0.75 | 0.285 |
| Total methylation rate | 85.93±0.45 | 85.66±1.81 | 0.463 |
Notes: Data are expressed as mean and standard deviation. The promoter region is 60 bp from 49 bp to 108 bp downstream of the CpG island transcriptional initiation codon (SIB). Total methylation rate is the C/C+T ratio at all positions from 744 bp upstream to 890 bp downstream from the CpG island transcriptional initiation codon. *P<0.05 (Pre vs Post).
Abbreviations: Pre, before TKA/UKA; Post, after TKA/UKA and exercise therapy for 5 months; PDK4, pyruvate dehydrogenase kinase 4; TKA, total knee arthroplasty; UKA, unicompartmental knee arthroplasty.