Figure 4.

Costaining for ATF4 or 4E‐BP1 with GFAP or OLIG2 demonstrate double positive cells of astrocyte but not oligodendrocyte lineage in VWM mice. ATF4‐GFAP costaining was only successful on sections from 2b4^he2b5^ho mice (4‐month‐old). Arrows indicate nuclei of double positive Bergmann glia. Shown are representative sections from a total of four sections (n = 2 animals per group). 4E‐BP1 + GFAP staining of astrocytes in cortex is at 2‐month‐old age. 4E‐BP1 + GFAP and 4E‐BP1 + OLIG2 double staining of cerebellar white matter (cb WM) is at an age of 12 months (HEP, 2b5^ho). Double positive astrocytes were prominent, whereas oligodendrocytes were not detected, even at HEP. Shown are representative sections from a total of 3–4 sections (n = 2 animals per group).