Fig. 4.

5-HT₇ antibodies do not discriminate between 5-HT₇ WT and KO tissue. One example of the failure of available 5-HT₇ receptor antibodies to detect differences in 5-HT₇ receptor expression between tissues from male WT and KO rats by immunohistochemistry in the isolated abdominal aorta (A) and vena cava (V) (A; Novus 46598) and Western analyses of cerebellar homogenates (B; Abcam 128892). Representative of at least three tissues (male and female) for each procedure. C: summary of all anti-5-HT₇ antibodies tested in WT and KO; location of epitope and assay used are indicated.