Table 1.

Primers used for genotyping, mutation validation, sequencing, and off-target analyses

Primer Name	Primer Sequence
O271_rHtr7_ex1_geno_F	CTGAGTGGCTTCCTAGAGGTGA
O272_rHtr7_ex1_geno_R	TTCCTTACCTGTCGATGCTGAT
O273_rHtr7_ex2_geno_F	TCTGCTAGGTACCTTGGGATCA
O274_rHtr7_ex2_geno_R	CAAGGTAGTGGCTGCTTTCTGT
O407_r5HT7_ex1WT_R	TCACCACCAGGCAGTTGCC
O428_r5HT7_ex1indel_R	TCACCACCTGCGATCGT
O409_rHtr1B_offtarget_F	ATGCGGTGGACTATTCTGCT
O410_rHtr1B_offtarget_R	AGAGCGGGCTTCCACATAGAT
O552_r5HT7-ex1_seq_F2	GAGCGGGCAAGGTGAATCCA
O553_r5HT7-ex1_seq_R2	GCTTGGTTGGTGTAGGGGTG
O554_r5HT7-ex2_seq_F1	CTGGCTGGTAGCACTCCGT
O555_r5HT7-ex2_seq_R1	TCTAGCCACTGATTCACGGGG
O566_r5HT7_cds-seq_F1	AGCGGGCAAGGTGAATCC
O567_r5HT7_cds-seq_R1	CCAGGGTTCCACTCTGGATCAT
O598_5-HT7_cDNA 3′ out seq F	CTGAGGACCACCTATCGTAGC

Referred to by name (e.g., O409) in the text and figure legends.