Fig. 6.

Effects of Stattic, a STAT-3 inhibitor, on STAT-3 activation and induction of inflammatory mediators in mouse lungs. Female C57BL/6 mice received vehicle or Stattic via intraperitoneal injections 16 and 1 h before intranasal administration of 50 μl of PBS or 20% dust extract (DE). After 3 h, mice were euthanized, and lungs were cleared of blood by perfusing with PBS and homogenized. A and B: levels of pSTAT-3, STAT-3, and actin were determined by Western blot analysis, and pSTAT-3 level was normalized to STAT-3, which had been normalized to actin. Data shown are means ± SE (n = 5 for PBS, n = 6 for DE and Stattic + DE). *P < 0.05, **P < 0.01 according to one-way analysis of variance using Tukey’s multiple-comparison test. C–F: levels of keratinocyte chemoattractant (KC), IL-1β, IL-6, and TNF-α were determined by ELISA and normalized to total protein levels. G: ICAM-1 levels were determined by Western blot analysis and normalized to actin levels. Data shown are means ± SE (n = 6–9). *P < 0.05, **P < 0.01, ****P < 0.0001; ns, not significant according to one-way analysis of variance using Tukey’s multiple-comparison test.