Figure 4.

GB and MW protective effect against oxidative stress induced by type 2 diabetes. (A a-d) Paraffin sections stained with anti-glutathione reductase antibody. The expression of glutathione reductase was increased in diabetic pancrease and back to almost normal after the use of GB and MW. Furthermore, (A e-h) Paraffin tissue sections stained with anti SOD2 antibody. SOD2 expression was intensively increased in diabetic pancreas while with the use of GB and MW was comparable with control. Scale bar 100 µm. Image J analysis displayed a significant increase of glutathione reductase and SOD2 intensities in diabetic pancreas compared to Ctrl. In Diabetic+GB and Diabetic+MW pancreas, glutathione reductase and SOD2 protein expression intensities were decreased to be comparable with Ctrl (B). *P<0.05, **P<0.01 and ***P<0.001 vs control group.

Abbreviations: GB, Ginkgo biloba; MW, magnetized water; Ctrl, control.