Table 2.
Quantitative PCR analysis of kidneys from 2K1C and sham mice
| Sham |
2K1C |
|||
|---|---|---|---|---|
| Cytokine | Unclipped-Left | Unclipped-Right | Unclipped-Left | Clipped-Right |
| IL-1β | 1.0 ± 0.3 | 1.1 ± 0.3 | 1.4 ± 0.3 | 7.3 ± 2.3*† |
| IL-2 | 1.0 ± 0.2 | 1.1 ± 0.3 | 1.1 ± 0.5 | 3.7 ± 0.7*† |
| IL-5 | 1.0 ± 0.3 | 0.8 ± 0.3 | 1.1 ± 0.3 | 0.9 ± 0.1 |
| IL-6 | 1.0 ± 0.3 | 0.4 ± 0.1 | 0.6 ± 0.1 | 2.9 ± 0.7*† |
| IL-10 | 1.0 ± 0.3 | 0.4 ± 0.1 | 0.8 ± 0.1 | 3.3 ± 0.8*† |
| IL-17F | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.3 ± 0.3 | 0.8 ± 0.3 |
| IL-23 | 1.0 ± 0.1 | 1.0 ± 0.1 | 2.3 ± 0.6 | 1.2 ± 0.1 |
| TNF-α | 1.0 ± 0.1 | 0.8 ± 0.1 | 0.8 ± 0.1 | 3.4 ± 1.0*† |
| MCP1 | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.1 ± 0.1 | 7.9 ± 2.0*† |
| Collagen 1 | 1.0 ± 0.1 | 1.1 ± 0.2 | 1.0 ± 0.1 | 5.3 ± 1.3*† |
| Fibronectin | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.1 ± 0.1 | 5.4 ± 1.3*† |
| TGF-β | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.2 ± 0.1 | 2.3 ± 0.3*† |
| IFN-γ | 1.0 ± 0.1 | 1.0 ± 0.1 | 1.4 ± 0.2 | 3.6 ± 0.6*† |
Values were normalized to β-actin and expressed as mean ± SE 2ΔΔCT in sham (n = 4) and 2-kidney,1-clip (2K1C; n = 4) mice. Statistical analysis was performed using 2-way ANOVA (group vs. kidney). MCP1, Monocyte chemoattractant protein 1; TGF-β, transforming growth factor.
*
P < 0.05, unclipped vs. clipped kidney in same animal.
†
P < 0.05, right unclipped sham vs. 2K1C-clipped kidney.