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. 2019 Aug 12;19:18. doi: 10.1186/s12861-019-0198-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — BMSCs viability and proliferation on cytodex 3. a BMSCs transfected with Shh or GFP viral plasmids revealed green fluorescence after 21 days of culture. GFP-transfected BMSCs treated with TGF-β served as a positive control. b Annexin V-PE immunofluorescence staining of BMSCs on cytodex 3 at day 21. Dead cells are labeled in red. c RT-PCR analysis of Annexin V on days 7,14 and 21 during differentiation induction. d, e Comparison of DNA and GAG/DNA ratio at different time points. MC, microcarrier. Significant differences between the control group are indicated by * p < 0.05, **p < 0.01