Fig. 3.

Mitochondrial NADH lifetime regulates glucose incorporation into acetyl-CoA and histone 4 lysine 16 acetylation. (A) Pseudocolored images mitochondrial NADH lifetime analysis using FLIM across cybrid heteroplasmy levels. (B) Quantification of mitochondrial NADH lifetimes (NAD⁺/NADH ratios) across cybrid heteroplasmy levels. (C) Conversion of ¹³C-glucose into acetyl-CoA across cybrid heteroplasmy levels. (D) ¹³C-glucose incorporation into H4K6 acetylation across cybrid heteroplasmy levels. (E) Relationship between NADH lifetime (NAD⁺/NADH) and ¹³C-glucose conversion to acetyl-CoA and H4K16 acetylation, showing strong correlation across cybrid heteroplasmy levels (Spearman), n = 3.