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. 2019 Jul 18;116(32):16121–16126. doi: 10.1073/pnas.1902321116

Fig. 3.

Fig. 3.

OsRac1 regulates cell division in rice panicle. (A) Quantitative real-time PCR analysis of cell cycle-related gene expression in the 4-cm-long young panicles of WT and OsRac1 transgenic lines. (B) Percentage comparison of the distribution of cells in different phases of the cell cycle in young panicle cells (the panicle length reached 5% of full panicle). The G1, S, and G2/M phases are shown in colored boxes. (C) The cell number (counts) that contain 2C DNA and 4C DNA in young panicles (the panicle length reached 5% of full panicle) of WT and OsRac1 transgenic lines by flow cytometry analysis. (D) Flow cytometry analysis of the young panicles at 3 developmental stages (the panicle length reached 5%, 10%, and 50% of full panicle) of WT and OsRac1 transgenic lines. The percentage of cells with 4C DNA content was determined as a proportion of the total number of isoploid and tetraploid nuclei. Means ± SD are shown in A (n = 3), B (n = 3), and D (n = 3). *P < 0.1; **P < 0.01; ***P < 0.001 (t test).