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. 2019 Jul 24;116(32):15967–15972. doi: 10.1073/pnas.1821315116

Fig. 2.

Fig. 2.

OsMTOPVIB is essential for bipolar spindle assembly. (A) The process of meiotic spindle assembly in OsmtopVIB. Chromosomes were stained with DAPI (blue), and microtubules were immunostained with α-tubulin antibody (green). (Scale bars: 5 μm.) (B) Products of meiosis in wild type and OsmtopVIB. In wild-type meiocytes, dyads at telophase I and tetrads at telophase II are shown. In OsmtopVIB, chromosomes are pulled to 2, 3, or 4 groups at telophase I. Tetrads, hexads, and octads are shown in OsmtopVIB at telophase II. (Scale bars: 5 μm.) (C) Frequencies of meiotic products in wild-type and OsmtopVIB meiocytes. At telophase I, 100% dyads are in wild type, while 24.27% dyads, 70.29% triads, and 5.44% tetrads are in OsmtopVIB (n = 515). At telophase II, 100% tetrads are in wild type (n = 505), while 14.16% tetrads, 19.30% pentads, 58.23% hexads, 2.12% heptads, and 6.19% octads are in OsmtopVIB (n = 565).