Fig. 3.

The mutation of OsREC8 suppresses multipolar spindle formation in OsmtopVIB. (A) Chromosome behavior in Osrec8 and Osrec8 OsmtopVIB meiocytes. (B) Biorientation of sister kinetochores in Osrec8 and Osrec8 OsmtopVIB. Centromeres were probed with CentO (red) through fluorescence in situ hybridization. Meiotic chromosomes were stained with DAPI (blue). (C) The process of meiotic spindle assembly in Osrec8 and Osrec8 OsmtopVIB meiocytes. Chromosomes were stained with DAPI (blue), and microtubules were immunodetected with α-tubulin antibody (green). (Scale bars: 5 μm.)