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. 2019 Jul 23;10:1662. doi: 10.3389/fimmu.2019.01662

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Copyright © 2019 Chen, Ma, Liu, Ma, Chen, Lin, Si, Ma and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gut microbiota intervention diminish Th17 responses in EAE. Lymphocytes from CNS, LN, small intestine, colon, and spleen were isolated 21 days post immunization and used for assessment of different CD4 T cell subsets. (A) Representative staining of different CD4 T cell subsets in CNS and LN, gated on TCRβ⁺ CD4⁺. (B) Statistical analysis of the percentages of IL-17 and IFN-γ in (A). Representative staining (D) and statistical analysis (C) of IL-17 and IFN-γ in CD4 T cells isolated from small intestine, colon, and spleen of mice, gated on TCRβ⁺ CD4⁺. Statistically significant data are indicated by asterisks (^**P < 0.01 and ^***P < 0.001). ns, not significant.