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. 2019 Jul 31;10:1739. doi: 10.3389/fimmu.2019.01739

Figure 2.

Figure 2

Induction of NETosis by PPARγ agonists. Human neutrophils from one control (A) and representative CGD (B) patient with homozygous delGT mutation were stimulated with 14 μg/μl of pioglitazone and 15 μg/μl of rosiglitazone for specified time intervals. Cells were treated with or without GW9662 along with PPARγ agonist treatment. Cells were then fixed with 4% PFA and stained with DAPI (nuclear stain) and antibodies against MPO and observed under confocal microscopy (63×). Scale bars, 20 μm. Images are representative of three independent experiments. *NETs formation was observed in a similar way in autosomal recessive and X-linked CGD cases and no difference was observed in both categories. (B) Representative image taken from a P2 patient with homozygous delGT.