Fig. 3.

Time course of male meiosis I in wild type. Anthers at meiotic stages were observed in plant expressing the TUBULIN BETA CHAIN 4 fused with the RED FLUORESCENT PROTEIN (RFP:TUB4) and the cohesin REC8 fused with the GREEN FLUORESCENT protein (REC8:GFP). Images were acquired every 3 min (Movie S1). The time is indicated on the snapshots in minutes, starting with the beginning of the movie. (A) Prophase. (B) Tubulin surrounds the nucleus at prometaphase. (C) The nucleus disappears and the spindle is forming. Metaphase I chromosomes are aligned in the spindle. (D) Early anaphase I, the REC8:GPF signal suddenly decreases. (E) Late anaphase I. The spindle has reorganized. (Scale bar, 10 µm.)