Fig. 5.

pDMC1::PANS1ΔD triggers arrest at metaphase II in spo11-1. Male meiocytes were spread and stained with DAPI. (A) Metaphase II in spo11-1. In this cell, three chromosomes are aligned on one plate, and seven on the other. (B) Metaphase II in spo11-1 pDMC1::PANS1ΔD. Similarly, uneven numbers of chromosomes are aligned on the two metaphase plates. (C) Anaphase II in spo11-1. Anaphase II with separation of sister chromatids (3, 3, 7, 7). (D) Pseudoanaphase II in spo11-1 pDMC1::PANS1ΔD with stretched chromosomes and no separation of sister chromatids. (Scale bars, A and B, 10 µm; D, 5 µm.)