Fig. 7.

Mutations in SEPARASE suppress pans1. (A) Nine-day-old plantlets of wild type, pans1-1 and pans1-1 esp-S606N grown on NaCl medium, as quantified in B. (B) Quantification of roots length of 9-d-old plantlets. (C) Metaphase II plates of wild-type, pans1-1, and pans1-1 esp-S606N plants as quantified in D. (D) Quantification of sister chromatid cohesion. Metaphase II plates were sorted into classes according to the number of single chromatids detected, from 0 to 10. Zero means full cohesion as observed in wild type; 10 indicates a complete loss of cohesion. The number of metaphase plates analyzed is indicated in parentheses. (Scale bars, A, 0.5 cm; C, 5 µm.)