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. 2019 Jul 22;116(32):16036–16045. doi: 10.1073/pnas.1902902116

Fig. 5.

Fig. 5.

Combination of human-like anti-Neu5Gc antibodies and Neu5Gc-rich HFD increases atherogenesis. (A) Cmah−/−Ldlr−/− male mice were immunized with either a control antigen (Ctl Immunization) or a Neu5Gc antigen (Gc Immunization) and then fed with a Neu5Ac-rich, Neu5Gc-rich, or non-Sias high-fat diet (Ac-HFD, Gc-HFD, and HFD, respectively) for 9 wk (n = 14 to 16, each). (B and C) Graphs representing body weight changes over 12 wk (HFD started at 9 wk of age, indicated by an arrow) in each group. En face analysis of atherosclerosis lesion (red dots and yellow arrows) in (D) control immunization and (E) Neu5Gc immunization groups. (Scale bars, 500 µm.) (F) Quantification of Sudan IV-positive area in aorta. Mean values ± SEM, *P < 0.05 and **P < 0.01.