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. 2019 Aug 5;10:868. doi: 10.3389/fphar.2019.00868

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Copyright © 2019 Lin, Chen, Zhang, Xu, Shao, Wang, Ding, Tian, Jin, Sheng, Gao, Lin, Zhang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of luteoloside on the cell viability of nucleus pulposus (NP) cells (A) Chemical structure of luteoloside. (B) The cytotoxic effect of 20 µM luteoloside on NP cells in a time-dependent manner using a Cell Counting Kit-8 (CCK-8) assay. (C) The cytotoxic effect of luteoloside on NP cells was determined at various concentrations for 24 h using a CCK-8 assay. (D) The cytotoxic effect of luteoloside on NP cells was determined at various concentrations with interleukin 1 beta (IL-1β) stimulation using a CCK-8 assay. (E) Morphological changes of NP cells treated with luteoloside in a dose dependent manner with IL-1β stimulation (original magnification × 200, scale bar: 50 µm). All experiments were performed at least three times, and the data in the figures represent the mean ± S.D. Significant differences between groups are indicated as **P < 0.01, *P < 0.05.