Critical Commercial Assays |
E.Z.N.A stool DNA kit |
Omega Biotek |
Cat#: D4015-02 |
AccuPrimeTaq DNA Polymerase, high fidelity kit |
Thermo Fisher Scientific |
Cat#12346086 |
High-sensitivity DNA analysis kit |
Agilent |
Cat#5067-4626 |
KAPA Library Quantification Kit for Illumina platforms |
KAPA Biosystems |
Cat# KK4873 |
SequalPrep Normalization Plate Kit, 96-well |
Thermo Fisher Scientific |
Cat#A1051001 |
500 cycle MiSeq V2 Reagent kit |
Illumina |
Cat#MS-102-2003 |
Deposited Data |
Raw and analyzed data |
This paper; Table S1
|
PRJNA531699 |
Experimental Models: Organisms/Strains |
Mouse: C57BL/6J |
The Jackson Laboratory |
Stock No: JAX: 000664 |
Mouse: C57BL/6NTac |
Taconic Biosciences |
Model: Black 6 (B6NTac), B6 |
Oligonucleotides |
16S rRNA gene Illumina sequencing primers |
Kozich et al., 2013 |
PubMed 23793624 |
Software and Algorithms |
GraphPad Prism version 7 |
GraphPad software |
https://www.graphpad.com/ |
Microsoft Excel |
Microsoft |
https://products.office.com/en-us/excel |
Mothur v.1.40.5 |
Schloss et al., 2009 |
https://www.mothur.org/ |
SILVA 16S rRNA database |
Pruesse et al., 2007 |
https://mothur.org/wiki/Silva_reference_files |
UCHIME |
Edgar et al., 2011 |
http://drive5.com/usearch/manual/uchime_algo.html |
16S rRNA gene training set version 9 of Ribosomal Database Project |
Wang et al., 2007 |
https://mothur.org/wiki/RDP_reference_files |
MeV |
Saeed et al., 2006 |
http://mev.tm4.org |
Past 3 |
Hammer et al., 2001 |
https://folk.uio.no/ohammer/past/ |
TreeGraph 2 |
Stöver and Müller, 2010 |
http://treegraph.bioinfweb.info/ |
GENE-E |
N/A |
https://www.broadinstitute.org/cancer/software/GENE-E/ |
Other |
PicoLab Laboratory Rodent Diet Irradiated 5L0D |
LabDiet |
Cat#: 0067138 |
Illumina MiSeq platform |
Illumina |
Illumina |