Figure 3.

Cardiac myocyte-specific Ribo-seq identifies myocyte translational regulation during cardiac stress by pressure overload. A, Experimental strategy for identification of the translatome and transcriptome during cardiac growth. B, Heart-weight-to-body-weight ratio (HW/BW) 3h, 2d, and 2w after transverse aortic constriction (TAC) surgery. N≥3 for each time point; *P<0.01. One-way ANOVA. C, Principal component analysis of RNA-seq and Ribo-seq libraries after TAC surgery. N≥3 for each time point. D, Translational or transcriptional control in response to TAC surgery. Transcripts were considered significant when false discovery rate <0.05 and Ribo-seq log₂-fold change of count per million >1 (upregulated) or <−1 (down-regulated). Venn diagram shows relative relationship between TAC 3h, TAC 2d, and TAC 2w regulation. N≥3 for each time point. E, Enrichment of gene ontology terms (biological process) in differentially expressed transcripts after TAC surgery. −log₁₀ P values, Fisher exact test. FC indicates fold change.